In Vitro Assays
SARS-CoV-2 pseudovirus neutralization assay
Antiviral Assay by CPE
- Cytopathic Effect (CPE) based assay is performed in Vero E6 cells; readout is Plaque Forming Units (PFU). Test compounds are checked for cytotoxicity in Vero E6 cells prior to the CPE assay.
- Assays can be performed with single concentration (for library testing), dose-response concentrations (IC-50) and drug combinations in checkerboard format.
Virus Yield Reduction Assay
- Assay is performed in mammalian cell lines (Calu-3, HEK293T, BHK21, A549); IC-50 is generated.
Virucidal Assay
- Testing of items such as sprays, disinfectants and compounds to test the direct killing/inactivation of the virus
Plaque Reduction Neutralization Test (PRNT) Assay
- PRNT assay is used for neutralizing antibody titer estimation in human or animal sera.
In Vivo Studies
- Anti-viral efficacy model for compounds in the Syrian Golden Hamster.
- Modes – therapeutic or prophylactic.
- Virus challenge model for vaccines and immunizing agents in the Syrian Golden Hamster.
Pharmacokinetics
- Pharmacokinetic evaluation of infected animals.
- Bioanalysis of infected samples.
Tester variants: SARS-CoV-2 tester strains available - wild type strain (Wuhan), variants of concern (alpha, beta, gamma and delta), other variants (Kappa, Scotland, Denmark & Germany)
Managed at partner BSL3 site