SARS-CoV-2 Studies

In Vitro Assays

SARS-CoV-2 pseudovirus neutralization assay

Antiviral Assay by CPE

  • Cytopathic Effect (CPE) based assay is performed in Vero E6 cells; readout is Plaque Forming Units (PFU). Test compounds are checked for cytotoxicity in Vero E6 cells prior to the CPE assay.
  • Assays can be performed with single concentration (for library testing), dose-response concentrations (IC-50) and drug combinations in checkerboard format.

Virus Yield Reduction Assay

  • Assay is performed in mammalian cell lines (Calu-3, HEK293T, BHK21, A549); IC-50 is generated.

Virucidal Assay

  • Testing of items such as sprays, disinfectants and compounds to test the direct killing/inactivation of the virus

Plaque Reduction Neutralization Test (PRNT) Assay

  • PRNT assay is used for neutralizing antibody titer estimation in human or animal sera.

In Vivo Studies

  • Anti-viral efficacy model for compounds in the Syrian Golden Hamster.
    • Modes – therapeutic or prophylactic.
  • Virus challenge model for vaccines and immunizing agents in the Syrian Golden Hamster.


  • Pharmacokinetic evaluation of infected animals.
  • Bioanalysis of infected samples.

Tester variants: SARS-CoV-2 tester strains available - wild type strain (Wuhan), variants of concern (alpha, beta, gamma and delta), other variants (Kappa, Scotland, Denmark & Germany)

Managed at partner BSL3 site