DMPK & Toxicology

Following the selection of preclinical candidates, Presude scientists can help you with a variety of in vitro and in vivo studies for DMPK, toxicity and bioanalytical validation.


Solution Properties

  • Solubility - Kinetic and Equilibrium
  • Log D 7.4
  • Chemical stability
  • Protein binding

Drug Metabolism

  • Metabolic stability (microsomes/S9)
  • CYP450 inhibition


  • Caco-2 permeation
  • PgP binding

In Vivo PK

  • Bioavailability
  • Plasma-brain ratio
  • Tissue Distribution
  • Excretion
  • Analysis in CSF (Rat)


  • Method development and validation on HPLC/LC-MS/MS. Ability to achieve sensitivity upto picogram level.
  • Bioanalytical support for GLP and regulated studies.
  • Fit-for purpose method development for N-GLP analysis.
  • Derivatization approach for poorly ionizable compounds.
  • Quantitation of analyte/ metabolites indifferent matrices plasma, serum, blood etc.
  • Method development in other matrices like urine, feces, bile, CSF, cell lysate and tissue homogenates.
  • PD marker identification for small molecules (Target based approach).
  • Development and validation of desired biomarkers for PK/PD
  • Quantitation of marker compounds in plasma of animals administered with plant extract for PK parameter evaluation.

Metabolite Identification

  • Metabolite generation using liver microsomes and plasma incubations
  • Metabolite generation using protocols of metabolic stability in liver
  • Microsomes and plasma stability at accelerated conditions
  • Chromatographic optimization for separation of all metabolites


In Vivo Toxicity Studies

  • Single Dose & MTD Range Finding Studies – Rat & Mice
  • Genotoxicity
  • Dermal Irritation Study
  • Ocular Irritation Study

Managed at partner site